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- www.med.nyu.edu
  
  E. coli Calcium Chloride competent cell protocol Then ...
  
  E. coli Calcium Chloride competent cell protocol. 1. Inoculate a single colony into 5mL Lb in 50mL falcon tube. Grow. O/N @ 37°C. 2. Use 1mL to inoculate ...
  
  2025-06-11
  
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- openwetware.org
  
  Preparing chemically competent cells - OpenWetWare
  
  Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating pipettor 5ml pipette Preparation Grow a 5ml overnight culture of cells i
  
  2025-06-11
  
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competent cells Methods, Protocols and Troubleshootings

日期：2025-06-14

Top10 and DH10B need IPTG for induction? - (reply: 1) Checking for efficieny competent cells - (reply: 1) freezing competent cells without dry ice or liquid N - (reply: 3) Expansion of commercial competent bacterial cell lines? - (reply: 2) What is your f...

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Protocol Competent cell preparation - iGEM 2010

日期：2025-06-11

Competent cell preparation. A. Preparing glassware and media eliminate detergent. 1. Autoclaving glassware filled 3/4 with DD-H2O to remove most detergent....

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Making Calcium Competent Cells

日期：2025-06-12

Streak out frozen glycerol stock of bacterial cells (Top10, DH5α, etc.) ... Prepare starter culture of cells ... autoclaved for the next time you make competent cells....

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E. coli Calcium Chloride competent cell protocol

日期：2025-06-14

E. coli Calcium Chloride competent cell protocol 1. Inoculate a single colony into 5mL Lb in 50mL falcon tube. Grow O/N @ 37 C. 2. Use 1mL to inoculate 100mL of LB in 250mL bottle the next morning. 3. Shake @ 37 C for 1.5-3hrs. Or 1. Inoculate a single .....

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Transformation of Competent Cells Protocol

日期：2025-06-14

Protocol on how to pour agar plates and do transformations. ... Biochemistry, Molecular Biology, and Cell Biology Protocols >> Transformation of Competent Cells Protocol for LB agar plates, add 15g/litre of Agar to the LB broth...

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