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日期:2024-04-27
The melting temperature of the amplicon at which the two DNA strands come apart is entirely predictable. ... HRM is a more cost effective solution and the high resolution element not only allows the determination of homo and heterozygosity, it also resolv...
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日期:2024-04-30
2012年7月12日 ... 所谓High Resolution Melting (HRM) analysis,就是改进了的DNA熔解曲线分析。其 原理就是DNA双链在加热过程中的解旋(熔解)动态与该DNA ......
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日期:2024-04-29
This protocol permits the mutation scanning of PCR products by high-resolution DNA melting analysis requiring the inclusion of a saturating intercalating dye in the PCR mix without labelled probe. During a scanning process, fluorescent melting curves of P...
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日期:2024-04-24
... SNPs are more amenable to high-throughput automated genotyping assays that allow samples to be genotyped faster and more ... Wirthensohn M, Gibson J, Ford C, Sedgley M. Mapping SNP-anchored genes using high-resolution melting analysis in2009; . ......
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日期:2024-04-29
melting curve acquisition Melting analysis was performed on the LightCycler or on a high-resolution melting instrument (HR-1; Idaho Technology), as described previously for labeled primers (10). After amplification, the samples were heated ......
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日期:2024-04-24
High-resolution DNA melting analysis for molecular diagnostics – REVIEW future science groupfuture ......
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日期:2024-04-24
High Resolution Melt (HRM) analysis is a powerful technique in molecular biology for the detection of .... the use of SYBR Green I for HRM, claiming that substantial protocol modifications are required....
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日期:2024-04-29
Real-Time PCR. 基本原理:. 隨著PCR反應循環逐次增加時,目標DNA也隨之快速增幅,此時. PCR反應液 ......