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日期:2025-04-25
This protocol describes the procedure for harvesting and re-plating RAW264.7 cells to maintain cultures at an appropriate cell density as avoid overgrowth and exhaustion of media. Protocol keywords: Passaging RAW264.7 RAW267.7 cell line Upload ......
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日期:2025-04-30
Tissue Culture- Sterile Passaging of RAW264.7 cells This video, intended for BIOL 315: Immunology at MSMU, demonstrates: aspirating media from a confluent dish of RAW cells, rinsing with PBS, aspirating the PBS, removing the cells from the dish surface us...
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日期:2025-04-29
RAW-Blue cells are derived from RAW 264.7 macrophages. They stably express a secreted embryonic alkaline phosphatase (SEAP) gene inducible by NF-kB and AP-1 transcription factors. Upon TLR, NOD or Dectin-1 stimulation, RAW-Blue cells activate NF ......
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日期:2025-04-30
PROPAGATION & CULTURING OF RAW264.7 CELLS Updated/Created By: Dawn Bowdish Date: February 14th, 2013 Bowdish Lab, McMaster University Hamilton, ON, Canada www.bowdish.ca BACKGROUND These cells are macrophage-like cell line derived ......
Transfection Protocol for Raw 264.7 (Mouse Leukaemic Monocyte Macrophage Cell Line) in Targefect Han
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日期:2025-04-29
The Targefect-Raw reagent is combined with the Virofect enhancer for optimal transfection of Raw 264.7 macrophages and other macrophage cell lines. The Targefect -Raw reagent enhances gene transfer by allowing the trasnfection complexes to escape degradat...
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日期:2025-04-29
siRNA into RAW264.7 cells? - any ideas (Oct/30/2008 ) Anyone had good success putting siRNA into RAW cells - if so what reagent/ratio did they use? I've tried several, none of them with overwhelming success.-Aaron I-QUOTE (Aaron I @ Oct 30 2008, 11:21 PM)...
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日期:2025-04-27
This line was established from a tumor induced by Abelson murine leukemia
virus....
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日期:2025-04-26
PROPAGATION &CULTURING OF RAW264.7 CELLS. Updated/Created By:
Dawn Bowdish Date: February 14th, 2013. Bowdish Lab, McMaster University....