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Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification

日期：2024-04-23

Droplet Digital PCR Instrumentation, Workflow, and Data Analysis All experimental work including ddPCR and droplet volume measurements was conducted at the National Measurement Institute, Australia. Reaction mixtures of 20 μL volume comprising 1× ddPCR .....

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Microfluidic Technologies for High Throughput

日期：2024-04-22

vii 3.5.2 Multilayer Soft Lithography: Elastomeric Valves 78 3.6 Detection: Development of a Fluorescence-Activated Droplet Sorter 85 3.7 Conclusions 92 4 Microfluidic Crossflow: Biochemical Screening ......

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Microfluidic Devices | Compare, Review, Quotes (RFQ) from Microfluidic Devices Manufacturers, Suppli

日期：2024-04-17

Dolomite, a world leader in the design and manufacture of microfluidic devices, has introduced the Micro Droplet Systems which enable rapid advances in droplet microfluidics allowing users to produce more than 10,000 monodispersed droplets per second. The...

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Controlled microfluidic interfaces : Article : Nature

日期：2024-04-17

Dispersed droplets may also be created using a microfluidic extension of Rayleigh's approach, with two streams of one liquid flanking a stream of a second immiscible liquid and the combined two-phase flow then forced through a small orifice. The pressure ...

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A Review of Heating and Temperature Control in Microfluidic Systems: Techniques and Applications | E

日期：2024-04-22

References 1. Bartlett, J.M.S.; Stirling, D. A short history of the polymerase chain reaction. Meth. Mol. Biol. 2003, 226, 3–6. 2. Maltezos, G.; Gomez, A.; Zhong, J.; Gomez, F.A.; Scherer, A. Microfluidic polymerase chain reaction. Appl. Phys. Lett. 2008,...

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Flow control in microfluidic devices | Elveflow microfluidic instruments

日期：2024-04-17

Home Products Elveflow® Microfluidic Flow Control Plug & Play instruments for high precision flow control in microfluidic devices OB1 Dual Vacuum & Pressure Controller AF1 Dual Vacuum & Pressure AF1 Pressure Pump MUX Flow Switches Matrix Flow Control ......

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Single-cell analysis and sorting using droplet-based microfluidics : Nature Protocols : Nature Publi

日期：2024-04-24

The fluorescence of each droplet is measured as the droplet flows past an observation constraint (OC) in the microfluidic channel in which the whole droplet is constrained to have a cross-section area of 20 × 25 μm as it passes an optical measurement fidu...

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Protein immobilization techniques for microfluidic assays

日期：2024-04-22

Planar microchannel surface In a large body of literature, protein is immobilized on microchannel surfaces made from silicon, glass, PDMS, plastic, or metal film deposited on channel surfaces. A protein monolayer can be formed on the surface after immobil...

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