search:reverse transcriptase pcr primer相關網頁資料

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日期:2025-12-17
One-step RT-qPCR only utilizes sequence-specific primers. In two-step assays, the reverse transcription and PCR steps are performed in separate tubes, with ......
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日期:2025-12-14
One-step RT-PCR reactions always employ a gene-specific primer for first-strand cDNA synthesis, while two-step RT-PCR reactions allow for other priming ......
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日期:2025-12-12
22 Jun 2009 ... If you are performing a one-step RT-PCR, gene specific primers are used because the RT primer is also your reverse primer for the PCR step....
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日期:2025-12-17
Hello, I would like to ask generally how you design your RT-PCR priemrs and specifically, how you check gene structure to contain some ......
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日期:2025-12-18
Methods Mol Biol. 2010;630:271-99. doi: 10.1007/978-1-60761-629-0_18. Primer design for RT-PCR. Li K(1), Brownley A. Author information: (1)J. Craig Venter ......
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日期:2025-12-14
Boston University. Biomedical Engineering Department. Biointerface Technologies Core. 44 Cummington Street. Boston, MA02218. Primer Design for RT-PCR....
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日期:2025-12-15
22 Sep 2012 ... Are the parameters same as that for normal PCR? Please explain in detail....