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日期:2024-04-24
One-step RT-qPCR only utilizes sequence-specific primers. In two-step assays,
the reverse transcription and PCR steps are performed in separate tubes, with ......
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日期:2024-04-22
One-step RT-PCR reactions always employ a gene-specific primer for first-strand
cDNA synthesis, while two-step RT-PCR reactions allow for other priming ......
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日期:2024-04-25
22 Jun 2009 ... If you are performing a one-step RT-PCR, gene specific primers are used
because the RT primer is also your reverse primer for the PCR step....
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日期:2024-04-27
Hello, I would like to ask generally how you design your RT-PCR priemrs and
specifically, how you check gene structure to contain some ......
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日期:2024-04-23
Methods Mol Biol. 2010;630:271-99. doi: 10.1007/978-1-60761-629-0_18.
Primer design for RT-PCR. Li K(1), Brownley A. Author information: (1)J. Craig
Venter ......
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日期:2024-04-27
Boston University. Biomedical Engineering Department. Biointerface
Technologies Core. 44 Cummington Street. Boston, MA02218. Primer Design for
RT-PCR....
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日期:2024-04-27
22 Sep 2012 ... Are the parameters same as that for normal PCR? Please explain in detail....