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![SuperScript III Reverse Transcriptase - Life Technologies]()
www.lifetechnologies.comSuperScript III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript II RT that is active at ...瀏覽：971 -
![Optimal Primer design for RT-PCR - Roche Life Science]()
lifescience.roche.comThe primer used for reverse transcription affects both the size and the specificity of the cDNA produced. Four kinds of primers are commonly used in RT-PCR, ...瀏覽：529
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日期：2025-06-01
One-step RT-qPCR only utilizes sequence-specific primers. In two-step assays,
the reverse transcription and PCR steps are performed in separate tubes, with ......
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日期：2025-06-04
One-step RT-PCR reactions always employ a gene-specific primer for first-strand
cDNA synthesis, while two-step RT-PCR reactions allow for other priming ......
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日期：2025-06-03
22 Jun 2009 ... If you are performing a one-step RT-PCR, gene specific primers are used
because the RT primer is also your reverse primer for the PCR step....
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日期：2025-06-05
Hello, I would like to ask generally how you design your RT-PCR priemrs and
specifically, how you check gene structure to contain some ......
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日期：2025-05-30
Methods Mol Biol. 2010;630:271-99. doi: 10.1007/978-1-60761-629-0_18.
Primer design for RT-PCR. Li K(1), Brownley A. Author information: (1)J. Craig
Venter ......
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日期：2025-06-06
Boston University. Biomedical Engineering Department. Biointerface
Technologies Core. 44 Cummington Street. Boston, MA02218. Primer Design for
RT-PCR....
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日期：2025-06-01
22 Sep 2012 ... Are the parameters same as that for normal PCR? Please explain in detail....
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