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日期:2025-05-11
http://www.abnova.com ) - TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ......
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日期:2025-05-07
Total RNA extraction using Trizol reagent. 1. Homogenization. 2. PHASE SEPARATION. Incubate the homogenized samples for 5 minutes at 15 to 30°C to ......
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日期:2025-05-10
the isolation of total RNA. Trizol is a mixture of guanidine thioacyanate and phenol, which effectively dissolves DNA, ... The same protocol can be used for RNA extraction from cell cultures. For further use of the RNA for expression analysis, it is highl...
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日期:2025-05-12
Hi, I've been trying to extract RNA from macrophages for an microarray study using different kits, but end up with unacceptably low RNA yields. The only method that gives acceptable yields is Trizol extraction, but I am worried about DNA contamination iss...
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日期:2025-05-07
Last week you isolated total RNA from tissue-cultured cancer cells using Trizol Reagent. Today you will purify the DNA and protein fractions. You will then determine the concentration and yield of the protein isolation in preparation for next week’s lab. ...
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日期:2025-05-09
2011年8月19日 - This is a protocol for extracting both RNA and DNA from mammalian tissue. It is currently written for tissue preparation but can also be used on ......
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日期:2025-05-10
RNA Extraction Protocol ... stored immediately following sacrifice and extraction! ... 2) At the same time, transfer at least 1mL TRIZOL / 100mg tissue to be ......
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日期:2025-05-07
TRIZOL Reagent facilitates isolation of a variety of RNA species of large or small .... removal of the aqueous phase, as described in the RNA isolation protocol,....