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Competent_Cells - Docstoc: Make Your Business Better
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日期：2026-05-06
... 10 mM KOAc pH 7.5 100 mM KCl 45 mM MnCl2 10 mM CaCl2 3 mM HexCo ... and further incubate on ice for 5' aliquot 0.2 ml of DMSO-treated competent cells into pre-cooled 1.5 ml Eppendorf tubes and shock-freeze competent cells in liquid N2 or ......看更多
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