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Lentiviral Transfection Protocol for a 10cm dish | McManus Lab
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日期:2026-04-26
*The day before, plate 3.0 x 10^6 293T cells 1. In 1.5 ml tubes mix 500ul SFMEM with 18 ul Mirus and incubate for 5 minutes at room temperature 2. In cap mix lentiviral plasmid with packaging vector master mix 3. Close lid and mix 4. Incubate 20 ......看更多
















