Making Calcium Competent Cells

Making Calcium Competent Cells

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日期:2025-12-02
100 mM CaCl2. 100 mM MgCl2. 85 mM CaCl2, 15% glycerol v/v. Centrifuge rotor . 3. Prepare starter culture of cells. Select a single colony of E. coli from fresh LB ......看更多