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Making Calcium Competent Cells - Molecular and Cell Biology Home
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日期：2024-06-13
Making Calcium Competent Cells Day 1 1. Streak out frozen glycerol stock of bacterial cells (Top10 ... Autoclave: 1 L LB (or your preferred media) 1 L of 100 mM CaCl2 1 L of 100 mM MgCl2 100 mL of 85 mM CaCl2, 15% glycerol v/v 4 centrifuge bottles and 2 2...看更多
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