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日期:2024-04-27
High resolution melt analysis (HRM analysis) is the quantitative analysis of the melt curves of product DNA fragments following PCR amplification. HRM can be ......
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日期:2024-04-25
The melting temperature of the amplicon at which the two DNA strands come apart is entirely predictable. ... HRM is a more cost effective solution and the high resolution element not only allows the determination of homo and heterozygosity, it also resolv...
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日期:2024-04-29
This protocol permits the mutation scanning of PCR products by high-resolution DNA melting analysis requiring the inclusion of a saturating intercalating dye in the PCR mix without labelled probe. During a scanning process, fluorescent melting curves of P...
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日期:2024-04-25
... SNPs are more amenable to high-throughput automated genotyping assays that allow samples to be genotyped faster and more ... Wirthensohn M, Gibson J, Ford C, Sedgley M. Mapping SNP-anchored genes using high-resolution melting analysis in2009; . ......
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日期:2024-04-26
melting curve acquisition Melting analysis was performed on the LightCycler or on a high-resolution melting instrument (HR-1; Idaho Technology), as described previously for labeled primers (10). After amplification, the samples were heated ......
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日期:2024-04-24
High-resolution DNA melting analysis for molecular diagnostics – REVIEW future science groupfuture ......
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日期:2024-04-26
2 Dec 2009 ... Basic Principles of HRM. • High Resolution Melting (HRM) is a relatively new PCR based method to ......
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日期:2024-04-28
High resolution melting curve analysis as a new tool for rapid identification of canine parvovirus type 2 strains Gali Bingga a, b, Zhicheng Liu b, Jianfeng Zhang b, Yujun Zhu c, Lifeng Lin b, Shuangyang Ding a, , , Pengju Guo d, , a Department of Veterin...