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日期:2025-04-27
High resolution melt analysis (HRM analysis) is the quantitative analysis of the melt curves of product DNA fragments following PCR amplification. HRM can be ......
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日期:2025-04-28
The melting temperature of the amplicon at which the two DNA strands come apart is entirely predictable. ... HRM is a more cost effective solution and the high resolution element not only allows the determination of homo and heterozygosity, it also resolv...
High Resolution Melting Analysis for fast and cheap polymorphism screening of marine populations : P
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日期:2025-04-26
This protocol permits the mutation scanning of PCR products by high-resolution DNA melting analysis requiring the inclusion of a saturating intercalating dye in the PCR mix without labelled probe. During a scanning process, fluorescent melting curves of P...
High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sati
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日期:2025-04-26
... SNPs are more amenable to high-throughput automated genotyping assays that allow samples to be genotyped faster and more ... Wirthensohn M, Gibson J, Ford C, Sedgley M. Mapping SNP-anchored genes using high-resolution melting analysis in2009; . ......
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melting curve acquisition Melting analysis was performed on the LightCycler or on a high-resolution melting instrument (HR-1; Idaho Technology), as described previously for labeled primers (10). After amplification, the samples were heated ......
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日期:2025-04-29
High-resolution DNA melting analysis for molecular diagnostics – REVIEW future science groupfuture ......
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日期:2025-04-28
2 Dec 2009 ... Basic Principles of HRM. • High Resolution Melting (HRM) is a relatively new
PCR based method to ......
High resolution melting curve analysis as a new tool for rapid identification of canine parvovirus t
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日期:2025-04-29
High resolution melting curve analysis as a new tool for rapid identification of canine parvovirus type 2 strains Gali Bingga a, b, Zhicheng Liu b, Jianfeng Zhang b, Yujun Zhu c, Lifeng Lin b, Shuangyang Ding a, , , Pengju Guo d, , a Department of Veterin...