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日期:2025-05-23
Established in 1978, UC San Diego Moores Cancer Center is one of just 41 National Cancer Institute-designated Comprehensive Cancer Centers in the United States, and the only one in the San Diego region. ... Transgenic Mouse: Gene Targeting General Outline...
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日期:2025-05-26
This protocol describes a culture system in which inner-ear sensory tissue is produced from mouse embryonic stem (ES) cells under chemically defined conditions. This model is amenable to basic and translational investigations into inner ear biology and re...
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日期:2025-05-24
Mouse embryonic stem cells (ESCs) can be transfected by electroporation, liposomal reagents, and viral transduction methods. The cationic polymer polyethylenimi ... Microscopy confirmed that transfections with PEI were feasible, but in order to quantify a...
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日期:2025-05-27
This protocol only gives an outline for mouse ES cell culture. Please refer to more
detailed protocols in the literature in ......
Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells : Nature Proto
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日期:2025-05-28
Each differentiation (diff.) stage is depicted with the number of days required for the differentiation, the medium with additives and a representative image. ESCs continue to self-renew indefinitely under the 2i + LIF condition. Images of PGCLCs, reconst...
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日期:2025-05-28
In general, human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) 1 can be cultured under variable conditions. However, it is not easy to establish an effective system for culturing these cells. Since the culture conditions ...
Stem Cell Research & Therapy | Full text | Efficient, high-throughput transfection of human embryoni
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日期:2025-05-27
Genetic manipulation of human embryonic stem cells (hESC) has been limited by their general resistance to common methods used to introduce exogenous DNA or RNA. Efficient and high throughput transfection of nucleic acids into hESC would be a valuable ......
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日期:2025-05-24
Some of the transfection efficiency and viability data was generated with the Microporator MP-100, the predecessor or the Neon Transfection System (see History of Neon ). For more up-to-date information, please perform a full-text...