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  Induced pluripotent stem cell - Wikipedia, the free encyclopedia
  
  Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell ...
  
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  Stem cell controversy - Wikipedia, the free encyclopedia
  
  The stem cell controversy is the ethical debate centered only with research involving the creation, usage, and destruction of human embryos. Most commonly, this controversy focuses on embryonic stem cells. Not all stem cell research involves the creation,
  
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Gene Targeting - Transgenic & Embryonic Stem Cell - UC San Diego Moores Cancer Center

日期：2024-04-29

Established in 1978, UC San Diego Moores Cancer Center is one of just 41 National Cancer Institute-designated Comprehensive Cancer Centers in the United States, and the only one in the San Diego region. ... Transgenic Mouse: Gene Targeting General Outline...

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3D mouse embryonic stem cell culture for generating inner ear organoids : Nature Protocols : Nature

日期：2024-04-26

This protocol describes a culture system in which inner-ear sensory tissue is produced from mouse embryonic stem (ES) cells under chemically defined conditions. This model is amenable to basic and translational investigations into inner ear biology and re...

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A simple and efficient method for transfecting mouse embryonic stem cells using polyethylenimine

日期：2024-04-30

Mouse embryonic stem cells (ESCs) can be transfected by electroporation, liposomal reagents, and viral transduction methods. The cationic polymer polyethylenimi ... Microscopy confirmed that transfections with PEI were feasible, but in order to quantify a...

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Optimized Protocol for Mouse Embryonic Stem Cells - Lonza

日期：2024-04-27

This protocol only gives an outline for mouse ES cell culture. Please refer to more detailed protocols in the literature in ......

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Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells : Nature Proto

日期：2024-05-01

Each differentiation (diff.) stage is depicted with the number of days required for the differentiation, the medium with additives and a representative image. ESCs continue to self-renew indefinitely under the 2i + LIF condition. Images of PGCLCs, reconst...

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Preparation of Mouse Embryonic Fibroblast Cells... | Protocol

日期：2024-04-30

In general, human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) 1 can be cultured under variable conditions. However, it is not easy to establish an effective system for culturing these cells. Since the culture conditions ...

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Stem Cell Research & Therapy | Full text | Efficient, high-throughput transfection of human embryoni

日期：2024-04-28

Genetic manipulation of human embryonic stem cells (hESC) has been limited by their general resistance to common methods used to introduce exogenous DNA or RNA. Efficient and high throughput transfection of nucleic acids into hESC would be a valuable ......

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Neon® Transfection System Protocols and Cell Line Data | Life Technologies

日期：2024-04-24

Some of the transfection efficiency and viability data was generated with the Microporator MP-100, the predecessor or the Neon Transfection System (see History of Neon ). For more up-to-date information, please perform a full-text...

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