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日期:2024-09-16
1. Anal Biochem. 1987 Apr;162(1):156-9. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Chomczynski P, Sacchi N. A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyan...
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日期:2024-09-18
2 the organic phase and reduces the formation of insoluble RNA-protein complexes at the interphase (24). Moreover, phenol retains about 10-15% of the aqueous phase, which results in a similar loss of RNA; chloroform prevents this retention of water and th...
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日期:2024-09-17
Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry. It is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases). This method may take...
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日期:2024-09-22
General Information Phenol/chloroform extraction is an easy way to remove proteins from your nucleic acid samples and can be carried out in a manner that is very close to quantitative. Nucleic acids remain in the aqueous phase and proteins separate into t...
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日期:2024-09-20
A non-phenol–chloroform extraction of double-stranded RNA from plant and fungal tissues Alitukiriza Balijja a, , , , Anders Kvarnheden b, Tullio Turchetti a a CNR, Istituto per la Protezione delle Piante, 50019 Sesto Fiorentino, Via Madonna del Piano 10, ...
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日期:2024-09-16
Dear all, as we use phenol-chloroform-isoamyl during DNA or RNA extraction and plasmid midi preparation, what is the actual mechanism to get rid of the protein by the phenol? is it related to the phenol molecule structure? Thanks ........
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日期:2024-09-21
( http://www.abnova.com ) - This method relies on phase separation by centrifugation of a mix of the aqueous sample and a solution containing phenol and chloroform, resulting in an upper aqueous phase and a lower organic phase (mainly chloroform). DNA is ...
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日期:2024-09-15
IntroductionGuidelinesMaterialsGeneral InformationIsolating Genomic DNATroubleshooting ... Add one volume of phenol:chloroform:isoamyl alcohol (25:24:1) to your sample, and vortex or shakeby hand thoroughly for approximately 20 seconds....