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日期:2024-04-27
2012年3月10日 - 實驗技術之原理介紹. 及實地操作課程. RNA ... RNA secondary and tertiary structure. 1. ... (2) 再以酸性之phenol/chloroform萃取,使RNA在水層....
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日期:2024-04-23
2 the organic phase and reduces the formation of insoluble RNA-protein complexes at the interphase (24). Moreover, phenol retains about 10-15% of the aqueous phase, which results in a similar loss of RNA; chloroform prevents this retention of water and th...
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日期:2024-04-23
General Information Phenol/chloroform extraction is an easy way to remove proteins from your nucleic acid samples and can be carried out in a manner that is very close to quantitative. Nucleic acids remain in the aqueous phase and proteins separate into t...
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日期:2024-04-27
Dear all, as we use phenol-chloroform-isoamyl during DNA or RNA extraction and plasmid midi preparation, what is the actual mechanism to get rid of the protein by the phenol? is it related to the phenol molecule structure? Thanks ........
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日期:2024-04-27
combination of guanidinium thiocyanate and hot phenol for RNA isolation was
reported by. Feramisco et al. in 1981 (14) ......
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日期:2024-04-28
12 Feb 2008 ... So that's how phenol extraction works. ... When i do the phenol:chloroform
extraction fo RNA, after the ......
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日期:2024-04-25
Phase extraction of nucleic acids is pH-dependent. At pH 7.0 and higher, RNA
and DNA will be solved in the aqueous....
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日期:2024-04-28
13 Sep 2010 ... Phenol/chloroform extraction is an easy way to remove proteins from your nucleic
acid samples and ......