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日期:2025-05-07
Transfection in multiwell plates — preparing a master mix If you are performing transfection in multiwell plates, prepare a master mix of transfection complexes or of transfection reagent and culture medium (depending on the protocol) for distribution int...
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日期:2025-05-03
A step-by-step guide of how to transfect COS-7 with TransIT-LT1 Transfection Reagent. For more information about TransIT-LT1, please visit https://www.mirusbio.com/products/tra......
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日期:2025-05-07
Learn more at http://www.lifetechnologies.com/trans... Optimized protocol for Lipofectamine LTX & Plus reagent: http://tools.invitrogen.com/content/s... For technical questions, please reach out to Technical Support at: 'techsupport@lifetech.com' or check...
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日期:2025-05-09
*The day before, plate 3.0 x 10^6 293T cells 1. In 1.5 ml tubes mix 500ul SFMEM with 18 ul Mirus and incubate for 5 minutes at room temperature 2. In cap mix lentiviral plasmid with packaging vector master mix 3. Close lid and mix 4. Incubate 20 ......
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日期:2025-05-09
American and European scientists used lentiviruses in a ground-breaking clinical trial aimed at curing adrenoleukodystrophy (ALD), a fatal brain disease in young boys that is caused by a mutated ALD gene. The lentivirus delivered a correct version of the ...
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日期:2025-05-09
Some-what large scale transfection - (reply: 2) Transient transfection trouble- massive cell death - (reply: 1) selection of transfected cells - (reply: 1) Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply: 3) too fe...
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日期:2025-05-03
Dharmacon RNAi, Gene Expression Gene Editing Protocol GE Healthcare DharmaFECT Transfection Reagents siRNA Transfection Protocol The following is a general protocol for use of Dharmacon DharmaFECT transfection reagents to deliver siRNA into ......
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日期:2025-05-08
Na+ H+ Wallert and Provost Lab Excel with hard work and inquiry 1 December 06 Basic Transfection Protocol Introduction: Transient transfection utilizes a mixture of liposomes and DNA to shuttle the plasmid DNA across the membrane. These protocols are ......