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An improved system for competent cell preparation and high efficiency plasmid transformation using d
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日期：2025-04-26
Competent cell preparation and plasmid transformation 116 Determination of their early log phase is important. The cells were pelleted by centrifugation at 8000 rpm for 1 min at 4ºC, then resuspended in one-half volume (20 ml) of sterile cold TB (CaCl2) s...看更多
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