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Competent cell preparation¬ using rubidium chloride
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日期：2025-10-19
Treat the competent cells gently as they are highly sensitive to handling and elevated temperature. 8. ... 30 mM potassium acetate 0.74g 10 mM CaCl2 1.25ml of 2M 50 mM MnCl2 2.47g 100 mM RbCl 3.023g 15% glycerol 37.5ml Adjust pH to 5.8 with 1M acetic ......看更多
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