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Freezing and Thawing Human Embryonic Stem Cells | Protocol
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日期：2025-12-11
1. Thawing hES cells Pre-experimental Set-up One day prior to thawing hES cells, plate a feeder layer of irradiated mouse embryonic fibroblasts (MEF), CF1 strain, on one well of a gelatin-coated 6-well tissue culture plate in MEF culture medium (D-MEM bas...看更多
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