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PREPARATION OF COMPETENT CELLS (E
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日期：2026-04-16
The original protocol from Amy uses 1L of E. coli culture. This protocol is scaled down to 100mL, and makes approximately 25 tubes of 200uL aliquots. Protocol: Note: STERILIZE EVERTHING (graduated cylinder and pipettes) 1. Grow E. coli in a 3mL LB broth ....看更多
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