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Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validatio
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日期:2024-04-25
1. Hum Mutat. 2009 Jun;30(6):899-909. doi: 10.1002/humu.21004. Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner. van der Stoep N, van Paridon CD...
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日期:2024-04-28
In the present study, we established a polymerase chain reaction (PCR)-coupled high resolution melting-curve (HRM) analysis method, utilizing the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA as the genetic marker, ......
BMC Genetics | Full text | A Strategy to Setup Codominant Microsatellite Analysis for High-Resolutio
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日期:2024-04-27
High resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA. The measurement is immediately following PCR in a one-step, closed-tu...
High-Resolution Melting Curve Analysis for Rapid Detection of Rifampin and Isoniazid Resistance... -
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日期:2024-04-23
FULL TEXT Abstract: We evaluated high-resolution melting (HRM) curve analysis as a tool for detecting rifampin (RIF) and isoniazid (INH) resistance in Mycobacterium... ... Extraction of DNA from the strains. All strains were subcultured onto a slant of L-...
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日期:2024-04-23
melting curve acquisition Melting analysis was performed on the LightCycler or on a high-resolution melting instrument (HR-1; Idaho Technology), as described previously for labeled primers (10). After amplification, the samples were heated ......
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日期:2024-04-25
High Resolution Melting (HRM) analysis is a relatively new, post-PCR ... A high-
resolution melt curve can be added to the end of the amplification run and ......
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日期:2024-04-23
HRM analysis differs from standard melt curve analysis in three ways: 1.
Chemistry—HRM ... High Resolution Melt Curve With a Single Point Mutation.
This is an ......