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      • www.kapabiosystems.com
        Application Guide High Resolution Melt Analysis Contents Page Introduction 3 Overview of the Melting Profile Principle 3 The Intercalating Dye Non-saturating dyes 4 Saturating dyes 4 Release-on-demand dyes 4 Instruments and Software 5 Summary of the ...
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      • www.bio-rad.com
        For HRM experiments, data are generally collected at narrower temperature increments than for standard melt curve protocols, ... Temesvári M et al. (2011). High-resolution melting curve analysis to establish CYP2C19∗2 single nucleotide polymorphism PMID:
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    日期:2024-04-25
    1. Hum Mutat. 2009 Jun;30(6):899-909. doi: 10.1002/humu.21004. Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner. van der Stoep N, van Paridon CD...
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    日期:2024-04-28
    In the present study, we established a polymerase chain reaction (PCR)-coupled high resolution melting-curve (HRM) analysis method, utilizing the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA as the genetic marker, ......
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    日期:2024-04-27
    High resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA. The measurement is immediately following PCR in a one-step, closed-tu...
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    日期:2024-04-23
    FULL TEXT Abstract: We evaluated high-resolution melting (HRM) curve analysis as a tool for detecting rifampin (RIF) and isoniazid (INH) resistance in Mycobacterium... ... Extraction of DNA from the strains. All strains were subcultured onto a slant of L-...
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    日期:2024-04-23
    melting curve acquisition Melting analysis was performed on the LightCycler or on a high-resolution melting instrument (HR-1; Idaho Technology), as described previously for labeled primers (10). After amplification, the samples were heated ......
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    日期:2024-04-25
    High Resolution Melting (HRM) analysis is a relatively new, post-PCR ... A high- resolution melt curve can be added to the end of the amplification run and ......
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    日期:2024-04-23
    HRM analysis differs from standard melt curve analysis in three ways: 1. Chemistry—HRM ... High Resolution Melt Curve With a Single Point Mutation. This is an ......