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1. Anal Biochem. 1987 Apr;162(1):156-9. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Chomczynski P, Sacchi N. A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyan...
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2 the organic phase and reduces the formation of insoluble RNA-protein complexes at the interphase (24). Moreover, phenol retains about 10-15% of the aqueous phase, which results in a similar loss of RNA; chloroform prevents this retention of water and th...
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Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry. It is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases). This method may take...
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A non-phenol–chloroform extraction of double-stranded RNA from plant and fungal tissues Alitukiriza Balijja a, , , , Anders Kvarnheden b, Tullio Turchetti a a CNR, Istituto per la Protezione delle Piante, 50019 Sesto Fiorentino, Via Madonna del Piano 10, ...
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IntroductionGuidelinesMaterialsGeneral InformationIsolating Genomic DNATroubleshooting ... Add one volume of phenol:chloroform:isoamyl alcohol (25:24:1) to your sample, and vortex or shakeby hand thoroughly for approximately 20 seconds....
Plant Methods | Full text | Protocol: A simple phenol-based method for 96-well extraction of high qu
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Consumables NOTE: If using alternative plastic ware, make certain it is resistant to phenol and chloroform. • 96-well racked 1.2 mL collection tubes and microtube caps (preferably in 8-tube strip format; QIAGEN, Cat.# 19560, #19566). • (Optional ......
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combination of guanidinium thiocyanate and hot phenol for RNA isolation was
reported by. Feramisco et al. in 1981 (14) ......
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12 Feb 2008 ... So that's how phenol extraction works. ... When i do the phenol:chloroform
extraction fo RNA, after the ......